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KMID : 0350519950480030867
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Journal of Catholic Medical College
1995 Volume.48 No. 3 p.867 ~ p.875
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Epithelial Viability of Intraperitoneal Trachea Allograft and Corticosteroid Therapy in Rats
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Abstract
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Airway anastomotic complications, especially ischemic injury, remain as a major cause of morbidity and mortality after clinical lung transplantation. There was a hypothesis that the effects of methylprednisolone (MP) impair the revascularization
and
epithelial regeneration of heterotropic syngeneic tracheal allografts. Therefore, the use of corticosteroid therapy to control pulmonary rejection has raised concern over delayed airway healing. This present study examined the effect of
methylprednisolone on the viability of the devascularized trachea after heterotrophic transplantation and omentopexy.
Thirty tracheal segments were harvested from 15 donor Sprague-Dawley rats, wrapped in omentum, and heterotropically implanted into the abdomen of recipient Wistar rats. Tracheal segments were randomly allocated into one of the five recipient
groups
(n=6) : receiving either no treatment, cyclosporine A (CsA) alone (15 mg/kg/day), CsA) in combination with MP (15 mg CsA+1 mg MP per kg/day or 15 mg CsA + 2mg MP per kg/day), or MP alone (2 mg/kg/day). After 14 days, the tracheal segments were
histologically evaluated.
Epithelial thickness and the degree of epithelial regeneration were signficantly different (p<0.05) between the immunosuppressed group and the untreated control Wistar rat group. Without immunosuppression there was virtually no epithelium,
whereas
immunosuppression with CsA, MP or CsA and low-dose MP yielded intermediate viability, and with CsA and the trachea exhibited significantly better regeneration after 14 days than it did in animals treated only with CsA.
We conclude that, in setting of heterotropic rat tracheal allografts treated with CsA, angiogenesis is not inhibited and epithelial regeneration is accelerated by MP treatment. These results indicate that, in heterotropic tracheal allografts,
viability
may be improved with an optimum combination of CsA and MP.
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